For the most part, no. You can wear hear down, makeup, jewelry, etc. The only time they made us put it up was for Dollie for a Day, and they weren't consistent with making us keep it that way throughout the day. So my recommendation would be to maybe put it up into a bun for Doolie for a Day just in case, but they might not make you keep it that way the whole time. Oh, here's one piece of unsolicited advice: if you hear rumor of what time they are planning to wake you up on Doolie for a Day, they will most likely wake you up half an hour earlier! We were all set to wake up half an hour earlier than when they were gonna get us up, and just as our phones went off to wake us, the cadre were there kicking and screaming at out door haha! Then we found ourselves outside in the hall five mins later doing PT before we could use the restroom or brush our teeth or anything, so if you want any time for that, I would try waking up as early as possible. Oh, and on Doolie for a Day, DON'T go anywhere alone without a wingman (I learned that the hard way lol!). Haha, sorry I know you just asked about the hair. I just thought I'd include a couple more things I wish I would have known going in haha!